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Tolerance of three marine microalgae to cryoprotectant dimethy sulfoxide, methanol and glycerol

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Title Tolerance of three marine microalgae to cryoprotectant dimethy sulfoxide, methanol and glycerol
 
Creator Joseph, Imelda
Panigrahi, A.
Chandra, P. Kishore
 
Description 243-247
Studies on
tolerance limit of cryoprotectants. is of much importance in determining the
viability of cells after freezing them at below normal temperatures. Tetraselmis
gracilis showed survival on exposure to 5 to 30% (v/v) DMSO, 5 to
25% methanol and 5 to 40% glycerol for periods of up to 2 h. DMSO and methanol
were lethal at higher concentrations (>10%)for Chlorella marina and Chaetoceros
calcitrans even on exposure for 15 min. At 25%, DMSO was so much lethal
that the cells of C. calcitrans and C. marina were
dead even after 60 sec. At 20%, all cells were dead within 60 min. Safer

concentrations for DMSO were
at 5% and lower. Methanol incubations significantly reduced cell viability
above 25% (60 sec) for T. gracilis, 15% (up to 20 min) for C. calcitrans
and 20% (up to 60 min) for C. marina. All the three algae could
tolerate glycerol even up to 30% (60 min). The survival was highest at 10%
concentration of glycerol for 120 min. Based on cell recovery rate after 24 h
post thaw and growth rate determination, DMSO at 5% or 10% glycerol are the
best treatments for cryopreservation. Glycerol was found to be better than DMSO
in all the three cases. Post thaw viability in 5% methanol was almost negligent
for all the three strains.
 
Date 2014-01-13T05:40:39Z
2014-01-13T05:40:39Z
2000-09
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/25525
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.29(3) [September 2000]