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Purification and characterization of Inulinase from marine bacterium, <i>Bacillus cereus</i> MU-31

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Title Purification and characterization of Inulinase from marine bacterium, Bacillus cereus MU-31
 
Creator Meenakshi, S
Umayaparvathi, S
Manivasagan, P
Arumugam, M
Balasubramanian, T
 
Subject Bacillus cereus
Extracellular inulinase
Fructose
Inulin
 
Description 510-515
Marine bacterium MU-31 strain isolated from
sediment of Vellar estuary Parangipettai (Southeast coast of India) was found
to secrete a large amount of inulinase into the medium. It was identified as
this marine bacterium potent isolated strain was identified as Bacillus
cereus by various biochemical studies. Optimum reaction conditions were as
follows: temperature, 30°C; pH,
7.0; NaCl, 2%; substrate concentration, 1.5%; carbon sources, inulin and
nitrogen sources, yeast extract. Purified enzyme gave a single band on gel
electrophoresis and its molecular weight was estimated to be 66 kDa. Among the
different chemicals used, only SDS enhanced the enzyme production and
1,10-phenanthroline was strongly inhibited the inulinase production. Among
metal ions, Mg2+ stimulated inulinase activity and strongly
inhibited by Hg2+ and Pb2+ at 1mM concentration. All
these conditions make Bacillus cereus MU-31 a potential candidate for
industrial enzymatic production of high fructose syrup and in other large-scale
biotechnological processes.
 
Date 2013-09-02T12:14:17Z
2013-09-02T12:14:17Z
2013-08
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/20901
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(4) [August 2013]