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Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of <i style="mso-bidi-font-style:normal">Spirulina platensis</i><span style="mso-bidi-font-family:"Times New Roman"" lang="EN-GB"> </span>

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Title Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis
 
Creator Shalaby, Emad A.
Shanab, Sanaa M. M.
 
Subject Spirulina platensis
Antioxidant
Antiradical
DPPH
ABTS
Phenolic compounds
 
Description 556-564
Present research was to evaluate and compare
the antiradical and antioxidant activities of extracts from
Spirulina platensis. In the present study, three extracts (Water,
absolute methanol and 50% methanol in water) were analyzed for the total phenolic
compounds, phycobiliprotein content: Antiradical and antioxidant activities
were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and
2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging
methods at 100 and 200 µg/mL. Results revealed that, absolute methanol
extract recorded the highest number of antiradical units 1 mg extract followed
in descending order by that of water while the lowest number was that of
aqueous methanol (against DPPH and ABTS radical methods) and these correlated
with chemical constituents of extract from phenolic and phycobiliprotein
compounds. It is elucidated that selection of the cyanobacterium (Spirulina
platensis) very important for consumer’s health, as it is considered as
potential sources of dietary antioxidants.
 
Date 2013-12-13T12:28:48Z
2013-12-13T12:28:48Z
2013-09
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/24794
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(5) [September 2013]