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Purification and activity of amylases of Marine <i style="mso-bidi-font-style:normal">Halobacillus </i><span style="mso-bidi-font-style:italic">sp<i style="mso-bidi-font-style:normal"> amylus</i> HM454199 </span>

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Title Purification and activity of amylases of Marine Halobacillus sp amylus HM454199
 
Creator Ashwini, K
Karthik, L
Kumar, Gaurav
Rao, K V Bhaskara
 
Subject Nicobar Islands
Halobacillus sp amylus
Optimization
Amylase
Microorganisms
Culture
 
Description 781-785
Present study was focused on purification
and activity of amylases of marine eubacteria. Among 23 bacterial isolates from
Nicobar Islands, Halobacillus sp amylus was selected and further
examined.  Enzyme activity was evaluated
at different temperatures (25, 30, 35, 40 and 45ºC), at varying pH (6.0, 6.5,
7.0, 7.5 and 8.0) and by using various carbon sources (starch, glucose,
sucrose, fructose, xylose and lactose). Different organic nitrogen sources (yeast
extract, meat extract, beef extract, nutrient broth, urea and casein) and
various concentration of NaCl were also used to evaluate enzyme activity. Maximum
enzyme activity was recorded at 40°C, pH
8.0 and 1.0% of substrate concentration.  Halobacillus sp amylus gave 12000 U of amylase per
litre of culture broth, 3 fold higher than before optimization in submerged
fermentation. Thus, these observations conclude that the enzyme obtained was
relatively heat sensitive and moderately alkalophilic amylase which can be
developed for extensive industrial applications.
 
Date 2013-12-13T12:46:48Z
2013-12-13T12:46:48Z
2013-10
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/24813
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(6) [October 2013]