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Isolation of aliphatic-antibiotic compounds from marine invertebrate, <i style="mso-bidi-font-style:normal">Heteractis</i><i style="mso-bidi-font-style:normal"><span style="mso-ansi-language:EN-IN"> magnifica</span></i> <span style="mso-bidi-font-size:15.0pt" lang="EN-GB">ʽQuoy & Gaimard, 1833ʼ against captive marine ornamental fish pathogens </span>

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Title Isolation of aliphatic-antibiotic compounds from marine invertebrate, Heteractis magnifica ʽQuoy & Gaimard, 1833ʼ against captive marine ornamental fish pathogens
 
Creator Gunasundari, V
Kumar, T T Ajith
Kumaresan, S
Balagurunathan, R
Balasubramanian, T
 
Subject Heteractis magnifica
Artemia nauplii
Hemolytic activity
Fish pathogen
Antimicrobial activity
 
Description 807-811
Present
study is aimed to screen and isolate the antibiotic compounds from the mucus of
Sea-anemone Heteractis magnifica. Mucus was extracted and characterized using gel chromatography,
Reverse Phase High Performance Liquid Chromatography and spectroscopic methods.
A molecular weight of purified protein was found to be 17KDa on SDS-PAGE.
Protein content of mucus recorded was 0.62mg/ml - Mucus extract exhibited
significant hemolytic activity in human, sheep, chicken and fish erythrocytes.
Median lethal concentration was determined using Artemia nauplii. Antimicrobial activity screening was performed against the ten isolated
bacterial pathogens and well inhibits the growth of Aeromonas
hydrophila, Flavobacterium sp.,
Pseudomonas fluorescens, Micrococcus sp., and Streptococcus sp.
The aliphatic nature of the mucus was determined by using FTIR
spectroscopic analysis. This is the first report on the aliphatic-antibiotic
compounds derived from Heteractis magnifica mucus which is used as marine ornamental fish disease
controlling agent.
 
Date 2013-12-13T12:32:30Z
2013-12-13T12:32:30Z
2013-10
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/24798
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(6) [October 2013]