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Screening and partial purification of antifungal metabolite from <i style="mso-bidi-font-style:normal">Streptomyces rochei</i> MSA14: an isolate from marine mining soil of Southwest coast of India.

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Title Screening and partial purification of antifungal metabolite from Streptomyces rochei MSA14: an isolate from marine mining soil of Southwest coast of India.
 
Creator Prakash, S.
Ramasubburayan, R.
Iyapparaj, P.
Kumar, C.
Mary, C. Jinitha
 
Subject Mining sediment
Antifungal activity
Streptomyces rochei
MIC and MFC
 
Description 888-897
A total of
fourteen actinobacterial strains were isolated from the mining sediment of
Manavalakurichi, Southeast coast of India. Primary screening results
through agar well diffusion method revealed that 28.57% actinobacterial strains
had in vitro antifungal activity. Most potent actinobacterial isolate MSA14
showed strongest inhibitory activity and was identified as Streptomyces rochei
through morphological, physiological, biochemical and 16S rRNA gene sequence
characteristics. Crude ethyl acetate extract of S. rochei exhibited wide
spectrum antifungal activity which was ranged between 12 and 17 mm. Further
evaluation of Minimum inhibitory concentration (MIC) and Minimum fungicidal
concentration (MFC) showed the values ranged from 50 to 200 and 100 to 200
µg/ml, respectively. Partial purification of crude extract through TLC using
various gradient solvent system recorded different spots of active principles
with the respective Rf values between 0.22 and 0.90. TLC autobiography assay
evidenced that, spot with the Rf value of 0.54 had promising antagonistic
activity.
 
Date 2013-12-13T13:00:27Z
2013-12-13T13:00:27Z
2013-11
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/24828
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.42(7) [November 2013]