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<i><span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">recA</span></i><span style="font-size:11.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Mangal; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">-based identification and antagonistic potential against fish pathogens by marine isolate DK1-SA11</span>

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Title recA-based identification and antagonistic potential against fish pathogens by marine isolate DK1-SA11
 
Creator Khan, Muhammad Naseem
Lin, Hong
Wang, Jingxue
Li, Meng
Mirani, Zulfiqar Ali
 
Subject Bacillus subtilis
Fish pathogens
recA gene
Antagonistic
Probiotics
 
Description 731-736
In this study, marine isolate DK1-SA11 was identified
and analyzed for antagonistic activity against fish pathogen along with its
potential possible role as probiotic agent. The strain DK1-SA11 was identified
on the basis of rec-A gene sequence similarity analysis and phylogenetic
studies. Strain DK1-SA11 antagonistic activity against fish pathogens was
analyzed by oxford cup agar diffusion method. Acute toxicity assay of strain
DK1-SA11 with zebra fish (Danio rerio) was performed to evaluate the
safety of strain for its future potential application as probiotic in
aquaculture. recA gene sequencing analysis identified the strain as Bacillus
sbutilis subsp. spizizenii strain DK1-SA11. Furthermore, strain
DK1-SA11 has shown broad antagonistic activity against fish pathogens including
Aeromonas hydrophila, A. salmonicida, Vibrio alginolyticus,

V. anguillarum, V. campbellii, V. chagasii, V. chagasii,
Photobacterium damsela, V. diazotrophicus, V. harveyi,
V. ichthyoenteri, V. mediterranei, V. mimicus, V.
parahaemolyticus, V. pomeroyi, V. splendidus, V. tubiashii,
V. vulniļ¬cus and V. xuii. Acute toxicity results revealed that
strain has neither fatal nor visible toxic effect to fish and the lethal
concentration (LC50) of DK1-SA11 to the fish was > 108
CFU mL-1. Therefore, it has been concluded that Bacillus subtilis
subsp. spizizenii strain DK1-SA11 might be a putative strain for
prevention and control of fish and shellfish diseases, and could be use for
probiosis.
 
Date 2016-06-23T11:30:47Z
2016-06-23T11:30:47Z
2014-05
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/28760
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.43(5) [May 2014]