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Prevention of Acyl homoserine lactone (AHL) mediated biofilm formation by selected flora of Andaman & Nicobar Islands

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Title Prevention of Acyl homoserine lactone (AHL) mediated biofilm formation by selected flora of Andaman & Nicobar Islands
 
Creator Deepa, S.
Venkateswaran, P.
Vinithkumar, N. V.
Kirubagaran, R.
 
Subject Acyl homoserine lactones
Anti-quorum sensing
Antifouling
Marine flora
 
Description 2101-2106
Acyl
homoserine lactones (AHL) are inter-cellular signal molecules and it controls
the population density dependent growth behavior of bacteria in a biofilm. The
AHL mediated biofilm formation and its inhibition was evaluated using methanol
(MeOH), ethyl acetate (EtOAc), n-butanol (n-BuOH) and aqueous (H2O) fractions
of 12 marine flora of Andaman and Nicobar Islands with fouling Vibrio sp., isolated from root surface
of mangrove. The 12 fractions were assayed for inhibition
of AHL promoted biofilm formation of Vibrio sp. and the results showed
that seven fractions possess AHL inhibitory potential. The extracts of
mangroves Avicennia marina, Rhizophora mucronata
and macroalgae Sargassum ilicifolium, Actinotrichia fragilis, Amphiroa anceps and Padina tetrastromatica
had anti-biofilm activity at least in one of the four solvent fractions.
Out of these, EtOAc fraction of Padina tetrastromatica
(60% inhibition at 50 µg) showed higher inhibition on AHL mediated antibiofilm
activity. The concentration dependent (10 - 200 µg) study of this fraction
showed that there was a significant reduction in biofilm formation by increase
in concentration with an IC50 (50 % inhibitory concentration)
of 40 µg. Thus it was concluded that the AHL mediated
biofilm inhibitory compound from Padina tetrastromatica can be used for
the development of anti-quorum sensing antifoulants.


 
Date 2016-06-30T05:22:34Z
2016-06-30T05:22:34Z
2014-11
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/34580
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.43(11) [November 2014]