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Calcium-Mediated Modulation of Staphylococcal Bacterial Biofilms

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Title Calcium-Mediated Modulation of Staphylococcal Bacterial Biofilms
 
Creator Shukla, Sudhir K.
Rao, T. Subba
 
Subject Staphylococcus aureus
Calcium
Biofilm
 
Description 2107-2117
Studies on the effect of calcium on Bap-mediated inhibition and
modulation of Staphylococcus aureus biofilm was the motivation
behind this study. In this study, the effect of calcium on modulation of Staphylococcus aureus
biofilm has been studied. Four S. aureus bovine mastitis isolates
viz. SA7, SA10, SA33 and bap-positive S. aureus V329 (positive control) along with a bap-isogenic
mutant M556 (negative control) were used in this study. PCR was used for
detection of bap, biofilm assays were done in microtitre plates and
confocal laser scanning microscopy (CLSM) was used to study the biofilm
architecture. PCR mediated detection identified SA7, SA10 and SA33 as bap-negative strains. Biofilm assay
showed that Ca2+ inhibited the biofilm growth of SA10 and SAV329 in
a dose dependent manner whereas SA7 and SA33 did not show any inhibition.
Strain M556 was found to be a weak biofilm former and showed no significant
change in the presence of Ca2+. The planktonic growth study results
showed that Ca2+ did not influence planktonic growth in any of the S. aureus strains except SA10 where ≥ 5
mM Ca2+ had an inhibitory effect. Interaction of Bap and Ca2+
was found to be specific, since studies with Mg2+ did not show any
inhibitory effect on V329 biofilm formation. CLSM images of V329, SA7 and SA10
biofilms showed reduction in biofilm thickness as well as altered biofilm
topography. The inhibition effect of Ca2+ on V329 and SA10 biofilms
disappeared in the presence of chelating agent EDTA at a sub-minimum inhibitory
concentration (0.15 mM). The paper elaborates the role of Ca2+ in
the biofilm architecture of S. aureus.
 
Date 2016-06-30T05:29:04Z
2016-06-30T05:29:04Z
2014-11
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/34581
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.43(11) [November 2014]