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Role of microbial aggregation in biofilm formation by bacterial strains isolated from offshore finfish culture environment

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Title Role of microbial aggregation in biofilm formation by bacterial strains isolated from offshore finfish culture environment
 
Creator Saravanan, N.
Verma, Pankaj
Mol, V. P. Limna
Kumar, R. Sendhil
Somasundaram, S. T.
Dharani, G.
Kirubagaran, R.
 
Subject Biofilm
Microcolony
Autoaggregation
Coaggregation
Growth phase
 
Description 2118-2129
Biofilm, the complex
aggregation of microbes encased in a multifaceted matrix, is a very important
module of marine biofouling. The biofilm formation is a sequential process
involving transport of microbes to a surface, initial attachment, formation of
microcolonies and biofilm maturation. Higher incidence of microcolonies, formed
by autoaggregation as well as coaggregation, leads to enhanced biofilm
formation. The present study was undertaken to elucidate the correlation
between microbial aggregation and biofilm formation by marine bacteria isolated
from the proximity of an offshore finfish cage. Seven bacterial strains were
isolated from High Density Poly Ethylene (HDPE) test coupons. Qualitative
analysis of auto and coaggregation was done using visual aggregation assay,
followed by light microscopic observation for further confirmation.
Quantitative analysis of aggregation was undertaken using Spectrophotometric
assay. Aggregation was monitored for bacterial cultures in lag, log and
stationary phases. The results indicated that the strain Oceanimonas smirnovii (NIOT-bflm-S10) exhibited maximum
autoaggregation during lag and log phase, while Salinicoccus roseus (NIOT-bflm-S12) exhibited maximum
autoaggregation during stationary phase. In case of coaggregation, O. smirnovii (NIOT-bflm-S10)–S. roseus (NIOT-bflm-S12), S. roseus (NIOT-bflm-S12) – Halomonas pacifica (NIOT-bflm-S13) and Pseudogracilibacillus
auburnensis (NIOT-bflm-S3) – O. smirnovii (NIOT-bflm-S10) exhibited
maximum aggregation index during lag, log and stationary phases, respectively.
Subsequently, O. smirnovii
exhibited maximum biofilm formation of 0.23, 0.26 and 0.42 (OD 595nm) after 24,
48 and 72 hours of incubation, respectively. In case of paired bacterial strains, maximum biofilm formation was
exhibited by O. smirnovii – S. roseus after 24 (0.450), 48 (0.370) and
72 hours (0.560) of incubation. Thus, factors responsible for microbial
aggregation, an immediate phenomena occurring in less than an hour of contact,
would be a better target for inhibiting biofilm formation. The antifouling
agent can be designed in such a way as to prevent / disrupt the initial
microbial aggregation.
 
Date 2016-06-30T05:42:43Z
2016-06-30T05:42:43Z
2014-11
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/34583
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.43(11) [November 2014]