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Antibiofilm activities of extracellular polymeric substances produced by bacterial symbionts of seaweeds

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Title Antibiofilm activities of extracellular polymeric substances produced by bacterial symbionts of seaweeds
 
Creator Viju, N.
Anitha, A.
Vini, S. Sharmin
Shankar, C.V. Sunjaiy
Satheesh, S.
Punitha, S. Mary Josephine
 
Subject Marine natural products
Surface associated bacteria
Extracellular polymeric substance
Antimicrobial activity
Adhesion
Biofilm
Antifouling
 
Description 2136-2146
In this study, 9 bacterial strains were collected
from the surface of seaweeds (Sargassum sp., Gracillaria sp. and Ulva sp.) and screened for their antibacterial
activity against three biofilm forming bacteria. All the 9 strains inhibited
the growth of biofilm-forming bacteria and the best active strains were
selected for in vitro and in vivo biofilm control assays. The results
showed that the extract of strain S8 controlled the biofilm formation
considerably. The toxicity of bacterial extracts was studied by determining the
LC50 value against Artemia sp.
The LC50 values obtained were found to be 0.061 and 0.105 mg/ml for
the extracts of strain S1 and S8 respectively. The best active strain S8 was
identified as Pseudomonas taiwanensis
based on 16S rRNA gene sequencing. The bioactive EPS was partially purified by
TLC and HPLC analysis. Besides, the functional groups present in the EPS was
analysed using FT-IR, which showed the presence of alcohol, alkenes, amines,
esters and carboxylic acid groups.
 
Date 2016-06-30T05:56:25Z
2016-06-30T05:56:25Z
2014-11
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/34585
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.43(11) [November 2014]