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<span style="font-size:15.0pt;mso-bidi-font-family:"Times New Roman";mso-bidi-font-weight: bold" lang="EN-GB">Screening, phenotypic and genotypic identification of β-carotene producing strains of <i style="mso-bidi-font-style:normal">Dunaliella</i> <i style="mso-bidi-font-style:normal">salina</i> from Thailand </span>

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Title Screening, phenotypic and genotypic identification of β-carotene producing strains of Dunaliella salina from Thailand
 
Creator Sathasivam, Ramaraj
Praiboon, Jantana
Chirapart, Anong
Trakulnaleamsai, Savitr
Kermanee, Prasart
Roytrakul, Sittiruk
Juntawong, Niran
 
Subject Screening
Morphology
RLFP
18S rDNA
ITS
rbcL gene
β-carotene
Dunaliella salina
 
Description 2198-2216
Dunaliella salina is a salt-loving microalga that accumulates
high amounts of β-carotene when cultivated under unfavorable conditions. In
this study we aimed to screen β-carotene producing strains of D.salina from salt soil samples collected from 19 provinces in the
northeastern part of Thailand. For preliminary screening 70 pure isolated
strains were screened by using 18S rDNA conserved primers (MA1&MA2), only
15 isolates produced a band (~2100 bp) as that of D.salina.  For the observation of β-carotene production, these 15 isolates were
grown on agar plate containing modified Ramaraj medium with 1.0 M NaCl under
continuous illumination.  After a
month only 3 isolates completely turned red. However, by using the
banding pattern produced by 18S rDNA primers, the 15 isolates could not be
differentiated between the β-carotene and non β-carotene producing strains.
Comparing the above results we found that the observation of β-carotene production on agar plate is more easy
and suitable method than molecular technique to screen β-carotene producing
strains. Morphological characteristics of the 3 screened Thai isolates clearly
delineated that it belongs to the genus Dunaliella.
ITS-RFLP banding pattern, 18S rDNA, ITS and RuBisCo large subunit (rbcL)
sequences were used to confirm at the species level. These results indicated
that these 3 isolates are D.salina and were named D.salina strain KU07, D.salina strain KU11 and D.salina strain KU13. Under non-stress
conditions these 3 strains had an ability to accumulate β-carotene up to
5.61±0.25, 7.58±0.19 and 6.73±0.32 pg/cell, respectively.


 
Date 2016-06-30T06:51:10Z
2016-06-30T06:51:10Z
2014-12
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/34591
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.43(12) [December 2014]