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<span style="font-size:15.0pt;line-height:115%;font-family:"Times New Roman"; mso-fareast-font-family:Calibri;mso-bidi-font-family:"Times New Roman"; mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-US">Some properties of C-phycocyanin from a native strain <i style="mso-bidi-font-style: normal">Anabaena affinis</i> isolated from Kovada Lake, Isparta, Turkey</span>

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Title Some properties of C-phycocyanin from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkey
 
Creator Akoglu, Aylin
Engin, Doruk
Cakmakci, M. Lutfu
 
Subject Phycocyanin
Cyanobacteria
Anabaena affinis
Purification
Antioxidant activity
 
Description 539-545
Some properties of C-Phycocyanin
(C-PC) from a native strain Anabaena
affinis isolated from Kovada Lake, Isparta,
Turkey were
discussed in the present. Purification
process was resulted in analytical grade product with 6.93-fold increase
in phycocyanin purity. By
using UV-VIS and fluorescence spectrophotometry, the highest absorbance and fluorescence were determined as
620 and 646 nm, respectively. SDS-PAGE analysis revealed the
presence of two major bands with a molecular mass of 17.1 and 18.6 kDa corresponding
to the α and β subunits and as confirmed by MALDI-TOF mass spectrometric
analysis.  Molecular mass of pure C-PC
was found as 156.2 and 198 kDa by HPLC gel filtration. Aggregation states of
C-PC were estimated as hexameric-(αβ)6 and trimeric forms-(αβ)3
along with two binding polypeptides of 27 kDa. High antioxidant activity was determined at 480 µM TEAC/mg phycocyanin
while no antimicrobial activity was detected.
 
Date 2016-07-06T11:03:02Z
2016-07-06T11:03:02Z
2015-04
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://hdl.handle.net/123456789/34734
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.44(04) [April 2015]