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The best DNA extraction method from sea anemone species with the mucosal tissue

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Title The best DNA extraction method from sea anemone species with the mucosal tissue
 
Creator Attaran-Fariman, Gilan
Javid, Pegah
 
Subject DNA Extraction
rDNA
Oman Sea
Modified PCI
Sea Anemone
Mucosal Tissue
 
Description 1011-1016
DNA extraction is the first and the most important step in genomic analysis of desoxy ribonucleique acid (DNA) for subsequent activities. DNA isolation is very difficult from sea anemones due to the mucosal contaminants. Different protocols are suggested for DNA extraction from sea anemones. The present study provides a DNA extraction protocol by some changing in common Phenol Chloroform method. This method was modified during the examination to achieve the best result. Sea anemones were collected from the southeast coasts of Iran, Oman Sea. They were transported to the laboratory and kept alive under in vitro conditions. The DNA was extracted by Phenol Chloroform/Isoamyl alcohol (PCI) method and extracted DNA was used as template for amplification in PCR. We proposed a modified PCI method in this study.
 
Date 2016-07-21T05:03:04Z
2016-07-21T05:03:04Z
2015-07
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/jspui/handle/123456789/34848
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.44(07) [July 2015]