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Apoptosis of SiHa and HCT116 cancer cell-lines by Scorpaenopsis venosa toxic extracts

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Title Apoptosis of SiHa and HCT116 cancer cell-lines by Scorpaenopsis venosa toxic extracts
 
Creator Sasikala, R.
Ravindran, Vinoth S.
 
Subject Chromatin condensation
FTIR
Hemolysis
Human cervical cancer cells (SiHa)
Human colon cancer cells (HCT)
MTT
SDS-PAGE
 
Description 155-160
Spine-venom gland and gonad of the scorpion fish Scorpaenopsis venosa was excised and extracted with Phosphate Buffer Saline. Crude protein was purified using DEAE cellulose anion exchange column chromatography. Protein content of the purified fractions of spine venom gland ranged from 0.053 to 0.076 mg.ml-1. Whereas, the protein content of the purified gonad extracts ranged from 0.056 to 0.076 mg.ml-1. Crude as well as the partially purified proteins had considerable haemolytic activity in chicken and goat
erythrocytes and it was found to be dose dependent. Fractionated extracts of the spine-venom gland induced cytotoxicity in Human cervical cancer cell line (SiHa) and Human colon cancer cell line (HCT 116). Purified fractions of spine-venom gland showed maximum apoptosis (59/100) in chromatin condensation assay against SiHa and HCT 116 (20.5/100). SDS-PAGE analysis of spine-venom gland fractionated protein yielded three bands at 71.2, 51 and 34 kDa and that of gonad yielded two bands one each at 40 and 35kDa. The FTIR analysis of the purified extracts showed peak values aligned as halide, amine, ether and alcohol.
 
Date 2016-07-21T06:01:56Z
2016-07-21T06:01:56Z
2016-01
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/jspui/handle/123456789/34885
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.45(01) [January 2016]