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Microbial Production of Astaxanthin pigment using Marine shrimp

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Title Microbial Production of Astaxanthin pigment using Marine shrimp
 
Creator Bhavsar, S. P.
Singh, K. G.
Sharma, L. P.
 
Subject Astaxanthin
Microbial fermentation
FFD
Chitosan
 
Description 352-357
In this study, shrimp waste collected from local hotels of Nashik and Trimbakeshwar, was used as a source of Astaxanthin and Lactobacillus spp. Overall, 4 isolates of Lactobacillus from various shrimp body parts viz., head, stomach, tail etc. were obtained and identified using morpho-biochemical characteristics. Astaxanthin extracted from dried shrimp waste using hexane/acetone gave 4% recovery (~0.4g/10g of shrimp) and microbial fermentation using Lactobacillus isolates showed enhanced astaxanthin production. Detection of the extracted pigment was done using thin layer chromatography (TLC). The concentrate was subjected to TLC using silica gel G250 sheet and run with solvent system of hexane:acetone (2:3). Thin layer chromatographic separation of carotenoid extract from shrimp waste produced a distinct band at Rf ~0.3 corresponding to free Astaxanthin. It was further characterized using spectrophotometry with absorption maxima at 465 nm and using High Performance Liquid Chromatography (HPLC). Preliminary study was done on microbial fermentation for astaxanthin production so as to select the efficient microbial culture and significant fermentation factors. This investigation concludes that Lactobacillus spp. are efficient candidate for astaxanthin fermentation and that soyabean extract supports maximum astaxanthin yield as indicated by the FFD .
 
Date 2016-07-22T08:36:27Z
2016-07-22T08:36:27Z
2016-02
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/35064
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Source IJMS Vol.45(02) [February 2016]