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Anti-MRSA potential of phenolic compound isolated from a marine derived actinomycete <i>Micromonospora</i> sp. ICN36

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Title Anti-MRSA potential of phenolic compound isolated from a marine derived actinomycete Micromonospora sp. ICN36
 
Creator Iniyan, Appadurai Muthamil
Joseph, Francisjoseph Rosemary Sharmila
Kannan, Rajaretinam Rajesh
Vincent, Samuel Gnana Prakash
 
Subject Marine actinomycete
Antibacterial resistance
Anti-MRSA agent
Agar plate fermentation
Small molecule
Time-kill assay
 
Description 1279-1287
Methicillin resistant Staphylococcus aureus (MRSA), the most frequently identified antimicrobial drug resistant pathogen is capable of evading the biocidal action of antibiotics and representing new paradigms in pathogenesis, transmission and resistance. There is a growing medical need for new anti-MRSA molecules. The present study is aimed to find a potent molecule from a marine derived actinomycete. Anti-MRSA compound producing Micromonospora sp. ICN36 was isolated and identified using morphological, physiological and 16S rDNA sequencing analysis. Extract was prepared from agar plate fermentation in the nutrient optimized media and the antagonistic molecule was chromatographically separated. UV-Vis, Infra red and liquid chromatography-mass spectral characterization was performed and the anti-MRSA potential of the molecule is reported. A phenolic compound with efficient anti-MRSA potential and a moderate cytotoxic property was isolated from the extract of ICN36. The anti- MRSA compound had absorbance at 215 nm with the molecular mass of 524.29 Da and exhibited 0.5 µg/ml MIC with rapid killing of MRSA in the in vitro time-kill assay. The anti-MRSA compound was found to be active against HCT15 colon carcinoma cell lines in vitro. Micromonospora sp. ICN36 from the marine sediment is considered as a potential anti-MRSA agent which is the important perspective of the present study.
 
Date 2016-10-18T06:15:45Z
2016-10-18T06:15:45Z
2016-10
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/35714
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.45(10) [October 2016]