Maintenance and optimization of culture/fermentation media to achieve maximum bioactive metabolites from marine <i>Strptomyces sp</i>. and their cytotoxic effects
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Title |
Maintenance and optimization of culture/fermentation media to achieve maximum bioactive metabolites from marine Strptomyces sp. and their cytotoxic effects
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Creator |
Haque, Md. Anwarul
Imam, Hasan Hana, Humayera Kabir Islam, Shaiful Ganim, Dilruba Haque, Md. Riazul |
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Subject |
Strptomyces sp.
Fermentation Optimization Bioactive metabolites Cytotoxicity |
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Description |
170-175
Marine soil and sediment samples were collected from different locations of mangrove forest Sundarbans, Bangladesh, the largest tidal halophytic mangrove forest in the world. On the basis of morphological, cultural and biochemical studies, the strain HAN-10 appeared as Streptomyces sp. Optimum cultural composition and fermentation parameters of this marine Streptomyces sp. was determined by the amount of metabolites released and consequently the inhibitory zone diameter measurement by disc diffusion method against Bacillus cereus. Maximum bioactive metabolites was observed when a broth medium containing glucose as carbon source, potassium nitrate, and 3.5% NaCl. Metabolites production was started after four days of incubation, reached its maximum levels after eight days, sustained upto ten days and thereafter gradually decreased. 37o C temperature and 6-7 pH was maintained for its optimum growth and metabolite release. |
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Date |
2017-03-02T05:50:21Z
2017-03-02T05:50:21Z 2017-01 |
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Type |
Article
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Identifier |
0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/40603 |
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Language |
en_US
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Rights |
CC Attribution-Noncommercial-No Derivative Works 2.5 India
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Publisher |
NISCAIR-CSIR, India
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Source |
IJMS Vol.46(01) [January 2017]
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