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Evaluation of the enzymatic profile of microbial isolates from Lonar Soda Lake

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Title Evaluation of the enzymatic profile of microbial isolates from Lonar Soda Lake
 
Creator Rathod, Mukundraj G.
Pathak, Anupama P.
 
Subject Alkaline protease
Bacillus
Alkaliphiles
Lonar crater
MEGA 6.06
 
Description 116-124
Alkaliphilic Gram positive bacteria were isolated and characterized from water and soil samples from alkaline Lonar lake, India. Total viable count of composite water and soil sample was found to be 1.4 × 105 cfu/mL and 1.7 × 107 cfu/gm, respectively. Thirty strains were isolated using different enrichment and isolation media. Alkaline protease assay was carried out to determine specific enzyme activity of partially purified alkaline protease. Ten alkaline protease producing isolates were identified as Bacillus subtilis LS-1, Bacillus clausii LS-2, Bacillus megaterium LS-3, Bacillus licheniformis LS-4, Bacillus thuringiensis LS-6, Bacillus alcalophilus LW9, Alkalibacillus sp. MGR8, Bacillus horikoshii MGR25, Micrococcus sp. MGR30 and Alkalibacterium sp. MGR11 on the basis of morphological, biochemical, physiological characterization, antibiotic sensitivity, enzyme profile and 16s rRNA sequencing. Out of these ten alkaline protease producers, nine isolates were belonged to phylum firmicute and one isolate belonged to phylum actinobacteria. Out of ten selected isolates, seven isolates were alkaline amylase producers. Two isolates were alkaline lipase and cellulase producers.
 
Date 2017-03-02T06:25:31Z
2017-03-02T06:25:31Z
2017-01
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/40610
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.46(01) [January 2017]