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On the accuracy of assessing copepod size and biovolume using FlowCAM and traditional microscopy

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Title On the accuracy of assessing copepod size and biovolume using FlowCAM and traditional microscopy
 
Creator Karnan, C.
Jyothibabu, R.
Kumar, T. M. Manoj
Jagadeesan, L.
Arunpandi, N.
 
Subject Plankton
Zooplankton
Copepod
Biovolume
Microscopy
FlowCAM
Arabian Sea
 
Description 1261-1264
In this paper, based on the biovolume estimation of different genera of copepods, we present the fact that only the Area-based Diameter (ABD) algorithm of FlowCAM has the efficiency to measure the bio-volume of copepods better than traditional microscopy. Also, we have demonstrated that the efficiency of the Equivalent Spherical Diameter (ESD) algorithm of FlowCAM over traditional microscopy is lesser while assessing the copepod biomass, and it depends on the morphological characteristics of various copepod genera. The ESD algorithm overestimates (8-140 times) while traditional microscopy method underestimates (2-8 times) the copepod biovolume, chiefly because of the inclusion of the entire image field for volume estimation in the former case and avoidance of extended body parts such as appendages in the latter case. These observations have special implications in aquatic environmental monitoring as many of the modern researchers prefer FlowCAM as a better tool to accurately quantify plankton biomass.
 
Date 2017-06-14T09:02:18Z
2017-06-14T09:02:18Z
2017-07
 
Type Article
 
Identifier 0975-1033 (Online); 0379-5136 (Print)
http://nopr.niscair.res.in/handle/123456789/42255
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJMS Vol.46(07) [July 2017]