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Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men

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Title Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men
 
Creator Kumar, R
Venkatesh, S
Kumar, M
Tanwar, M
Shasmsi, M B
Kumar, R
Gupta, N P
Sharma, R K
Talwar, P
Dada, Rima
 
Subject Reactive oxygen species
Oxidative stress
Infertility
Antioxidant enzymes
mtDNA mutations
Assisted reproductive technique
Sperm
Oligoasthenozoospermic
 
Description 172-177
Physiological function of reactive oxygen species (ROS) has been known since a long, but recently toxic effects of ROS on spermatozoa have gained much importance in male infertility. Mitochondrial DNA (mtDNA) is believed to be both source and target of ROS. mtDNA unlike nuclear DNA is not compactly packed and hence more susceptible to oxidative stress (OS) than nuclear DNA. In the present study, the role of OS in mitochondrial genome changes was studied in men with idiopathic infertility. The study included 33 infertile oligo-asthenozoospermic (OA) men and 30 fertile controls. Semen analyses were performed and OS was measured by estimating the level of malondialdehye (MDA) in the seminal plasma and ROS in the sperm. Sperm mtDNA was sequenced by standard PCR-DNA sequencing protocol for ATPase and nicotinamide adenine dinucleotide dehydrogenase (ND) groups of genes. Sperm count and progressive motility were found to be significantly lower in infertile group than the fertile controls. Semen MDA and ROS levels of infertile group were significantly higher (p
 
Date 2009-05-01T04:16:48Z
2009-05-01T04:16:48Z
2009-04
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/4054
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(2) [April 2009]