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Optimization of physical parameters for lipase production from <i style="">Arthrobacter</i> sp. BGCC#490

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Title Optimization of physical parameters for lipase production from Arthrobacter sp. BGCC#490
 
Creator Sharma, Anjana
Bardhan, Dipa
Patel, Rashmi
 
Subject Alkaline lipase
Arthrobacter sp.
Face centered central composite design
Response surface methodology
Statistical analysis
 
Description 178-183
The physical parameters for the production of thermostable, alkaline lipase from Arthrobacter sp. BGCC# 490 were optimized using response surface methodology (RSM), employing face centered central composite design (FCCCD). The design was employed by selecting pH, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by RSM. A second-order quadratic model and response surface method showed that the optimum conditions for lipase production (pH 10.0, temperature 40oC and incubation period 48 h) resulted in 1.6-fold increase in lipase production (13.75 EUml-1), as compared to the initial level (8.6 EUml-1) after 48 h of incubation, whereas its value predicted by the quadratic model was 12.8 EUml-1. Lipase showed stability in the pH range 8-10 and temperature range 40-60oC, with maximum activity at pH 9.0 and temperature 50oC. Lipase activity was enhanced in the presence of K+, Ca2+ and Mg2+ ions, but inhibited by Hg2+ ions. The enzyme exhibited high activity in the presence of acetone, isopropanol and ethanol, but was unaffected by methanol. These properties suggest that the lipase may find potential applications in the detergent industry. The present work also demonstrated the feasibility of using experimental design tools to optimize physical parameters for lipase production by an indigenous Arthrobacter sp.
 
Date 2009-05-01T04:17:02Z
2009-05-01T04:17:02Z
2009-04
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/4055
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(2) [April 2009]