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Properties of alkaline protease genetically engineered on cell surface of the yeast <i style="">Yarrowia lipolytica</i>

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Title Properties of alkaline protease genetically engineered on cell surface of the yeast Yarrowia lipolytica
 
Creator Ni, Xiumei
Yue, Lixi
Li, Jing
Chi, Zhenming
Liu, Zhiqiang
Madzak, Catherine
 
Subject Yeast surface display
Alkaline protease
Aureobasidium pullulans
Yarrowia lipolytica
Marine yeast
 
Description 294-298
ALP2 gene encoding alkaline protease cloned from Aureobasidium pullulans HN2-3 was ligated into the surface display plasmid and expressed in the cells of the yeast Yarrowia lipolytica. The expressed alkaline protease was immobilized on the yeast cells. The activity of the immobilized enzyme with 6 His tag was found to be significantly higher than that of without 6 His tag. The immobilized enzyme showed lower optimal temperature and a lower affinity for azocasein than the free enzyme purified from A. pullulans HN2-3. The thermal stability of the immobilized enzyme enhanced and the pH stability decreased, compared to that of the free enzyme.
 
Date 2009-08-06T11:14:56Z
2009-08-06T11:14:56Z
2009-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/5797
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(4) [August 2009]