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Identification of <img src='/image/spc_char/alpha.gif'><sub>2u</sub>-globulin and bound volatiles in the Indian common house rat (<i>Rattus rattus</i>)

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Title Identification of 2u-globulin and bound volatiles in the Indian common house rat (Rattus rattus)
 
Creator Rajkumar, R
Ilayaraja, R
Mucignat, C
Cavaggioni, A
Archunan, G
 
Subject 2u-Globulin
Major urinary protein
Mass spectrometry
Pest control
Pheromone-binding protein
Rattus rattus
Urine
 
Description 319-324
The 2u-globulin (2u) is a pheromone carrier urinary protein believed to be relevant for sexual communication among rats and is characterized in laboratory rats. In the present study 17 kDa protein and the bound pheromones were characterized in a population of wild-type Indian common house rat (Rattus rattus). The protein was purified by two runs of Sephadex G-50 chromatography and analyzed with SDS-PAGE with MALDI-TOF/MS. The results of MASCOT search identified the protein as an 2u and suggested a role for binding pheromones. To confirm the protein bound volatiles, purified 2u was extracted with dichloromethane and volatile molecules were detected using of gas chromatography linked to mass spectrometry (GC-MS). 1-Chlorodecane was detected as the predominant compound and 2-methyl-N-phenyl-2-propenamide, hexadecane and 2,6,11-trimethyl decane as the minor compounds. The simple method of protein purification and the identification of bound volatiles may help in designing efficient pheromone-based rat traps.
 
Date 2009-08-06T11:51:24Z
2009-08-06T11:51:24Z
2009-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/5800
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(4) [August 2009]