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Purification and characterization of bacteriocin produced by strain of <i style="">Lactobacillus brevis</i> MTCC 7539

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Title Purification and characterization of bacteriocin produced by strain of Lactobacillus brevis MTCC 7539
 
Creator Gautam, Neha
Sharma, Nivedita
 
Subject Bacteriocin
Lactibacillus brevis
 
Description 337-341
Bacteriocin, an antimicrobial agent having potential for food biopreservation was purified from Lactobacillus brevis (a safe food-grade bacteria isolated from Vari Kandal, a traditional fermented food of Himachal Pradesh by adopting a novel repeated washing method. Its purity was confirmed by SDS-PAGE and Native-PAGE. The relative molecular mass of bacteriocin was 93.74 kD, while specific activity and recovery were 35.52 folds and 17.13%, respectively. It showed high thermal stability and was active over wide range of pH and exhibited sensitivity to trypsin.
 
Date 2009-08-06T11:53:48Z
2009-08-06T11:53:48Z
2009-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/5803
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(4) [August 2009]