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Efficient and rapid purification of lentil <img src='/image/spc_char/alpha.gif'>-galactosidase by affinity precipitation with alginate
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View Archive Info| Field | Value | |
| Title |
Efficient and rapid purification of lentil  -galactosidase by affinity precipitation with alginate
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| Creator |
Çelem, Evran Bıçak
Bolle, Sharon Sibel Önal, Seçil |
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| Subject |
Affinity macroligand
Affinity precipitation Alginate -Galactosidase
Lentil seed Purification |
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| Description |
366-370
-Galactosidase (-D-galactoside galactohydrolase, EC 3.2.1.22) was purified (26-fold) from the germinating seeds of lentil (Lens culinaris) by affinity precipitation with alginate. The purified enzyme gave a single band corresponding to molecular mass of 40 kDa on SDS-PAGE. The optimum temperature and pH of the enzyme were determined as 40oC and 5.5, respectively. The enzyme was very stable at a temperature range of 4-65oC and at a pH range of 4-7. The values of kinetic constants Km and Vmax using p-nitrophenyl--D-galactopyranoside (PNPG) as substrate were 0.191 mM and 0.73 U, respectively. Results suggest that affinity precipitation is an attractive process for the purification of -galactosidase.
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| Date |
2009-10-28T03:55:12Z
2009-10-28T03:55:12Z 2009-10 |
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| Type |
Article
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| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/6293 |
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| Language |
en_US
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| Publisher |
CSIR
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| Source |
IJBB Vol.46(5) [October 2009]
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