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Oxidation of oxymyoglobin by poplar plastocyanins <i style="">a</i> and <i style="">b</i>

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Title Oxidation of oxymyoglobin by poplar plastocyanins a and b
 
Creator Christova, Petya K
Donchev, Anthony A
Shosheva, Alexandra Ch
Getov, Vladimir I
Dimitrov, Mitko I
 
Subject Dimorphism
Photosynthesis
Electron transfer
pH-dependence
Ionic strength dependence
 
Description 383-388
Oxidation of oxymyoglobin [MbO2 (Fe2+)] by isoplastocyanins a (PCa) and b (PCb) was experimentally investigated and the corresponding redox reaction was modeled using the physicochemical parameters of the isoforms to study the effect of the dimorphism. The kinetic curve of oxidation of MbO2 (Fe2+) by oxidized PCa [PCa(Cu2+)] and PCb [PCb(Cu2+)] and the pH-dependence of the rate constant k1 were determined. In the range of pH 4.8-9.0, PCb reacts with higher k1, compared with PCa. For example, at pH 7.0, k1(PCb) = 4 × 102 M-1s-1, whereas k1(PCa) = 2 × 102 M-1s-1. The observed values of ΔE0 for the reaction pairs Mb-PCa and Mb-PCb were -304 mV and -319 mV, respectively. The effect of the ionic strength (µ) on the rate of the electron transfer was also studied. It was found that: (i) the net charge Z1 of PCa and PCb fully corresponds to that calculated by their primary structures and Z2 of Mb corresponds to that calculated by its titration curve; (ii) the ln k as function of √¯µ was similar for both PCa and PCb; (iii) the curve of the reaction PCb Mb (pH 7.0) was shifted towards higher values of k, in agreement with the larger net negative charge of PCb; and (iv) the character of the electrostatic interactions remained unchanged by a replacement of PCa by PCb and by the change of pH from 7.0 to 4.8.
 
Date 2009-10-28T03:56:03Z
2009-10-28T03:56:03Z
2009-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/6296
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(5) [October 2009]