Record Details

Kinetics of oxidation of adenosine by <i>tert</i>-butoxyl radicals: Protection and repair by chlorogenic acid

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Kinetics of oxidation of adenosine by tert-butoxyl radicals: Protection and repair by chlorogenic acid
 
Creator Vijayalakshmi, G
Adinarayana, M
Rao, P Jayaprakash
 
Subject Chlorogenic acid
Adenosine repair
t-BuO. radicals
Oxidation
 
Description 389-394
The rates of oxidation of adenosine and chlorogenic acid by tert-butoxyl radicals (t-BuO-) were studied by measuring the absorbance of adenosine at 260 nm and chlorogenic acid at 328 nm spectrophotometrically. t-BuO- radicals were generated by the photolysis of tert-butyl hydroperoxide (t-BuOOH) in presence of tert-butyl alcohol to scavenge OH. radicals. The rates and the quantum yields() of oxidation of chlorogenic acid by t-BuO-radicals were determined in the absence and presence of varying concentrations of adenosine. An increase in the concentration of adenosine was found to decrease the rate of oxidation of chlorogenic acid, suggesting that adenosine and chlorogenic acid competed for t-BuO. radicals. From competition kinetics, the rate constant of chlorogenic acid reaction with t-BuO- was calculated to be 3.20 109 dm3 mol-1 s-1. The quantum yields (expt) were calculated from the experimentally determined rates of oxidation of chlorogenic acid under different experimental conditions. Assuming that chlorogenic acid acts as a scavenger of t-BuO- radicals only, the quantum yields (cal) were theoretically calculated. expt and cal values suggested that chlorogenic acid not only protected adenosine from t-BuO- radicals, but also repaired adenosine radicals, formed by the reaction of adenosine with t-BuO- radicals.
 
Date 2009-10-28T03:56:21Z
2009-10-28T03:56:21Z
2009-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/6297
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(5) [October 2009]