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Novel multi-layer APPPA microcapsules for oral delivery: preparation condition, stability and permeability

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Title Novel multi-layer APPPA microcapsules for oral delivery: preparation condition, stability and permeability
 
Creator Ouyang, Wei
Chen, Hongmei
Jones, Mitchell L
Haque, Tasima
Martoni, Christopher
Afkhami, Fateme
Prakash, Satya
 
Subject Microcapsule
APPPA
Membrane Strength
GI stability
Artificial Cells
Oral Delivery
Pectin
 
Description 491-497
Oral therapy utilizing cell microencapsulation has shown promise in the treatment of many diseases. Current obtainable microcapsule membranes, however, show inadequate stability in the gastrointestinal (GI) environment, thus restricting the general application of live cells for oral therapy. To overcome this limitation, we have previously developed a novel multi-layer alginate/poly-L-lysine/pectin/poly-L-lysine/alginate microcapsule (APPPA) with demonstrated improvement on membrane stability over the frequently reported alginate/poly-L-lysine/alginate (APA) microcapsules. In this study, we further examined the effects of preparation conditions on microcapsule formation, and assessed the membrane strength and GI stability. Results showed that increased membrane strength of the APPPA microcapsules was attained by using pectin with low degree of esterification as the mid-layer material, saline as the solvent for the preparation solutions and washing medium, and 0.1 M CaCl2 as the gelling solution for alginate cores. Resistance of this membrane to the simulated GI fluids was also investigated. Permeability of and release profiles from the APPPA microcapsules were found comparable to the APA microcapsules. These findings suggested that the multi-layer APPPA microcapsule formulation may have potential in oral delivery of proteins, live bacterial cells and other biomedical applications.
 
Date 2010-01-25T05:46:34Z
2010-01-25T05:46:34Z
2009-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/7252
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.46(6) [December 2009]