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DNA damage by ribose: Inhibition at high ribose concentrations

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Title DNA damage by ribose: Inhibition at high ribose concentrations
 
Creator Waris, Sahar
Pischetsrieder, Monika
Saleemuddin, Mohammed
 
Subject Ribose
DNA glycation
DNA-AGEs
 
Description 148-156
Nucleobases and DNA react non-enzymatically
with sugars, and generate DNA-advanced glycation end products (DNA-AGEs).
Incubation of plasmid pBr322 with ribose for 3-7 days caused the transformation
of the supercoiled plasmid to the open circular and linear forms. Removal of
sugar after an initial 24 h incubation resulted in marked enhancement in the transformation
rate. Enhancement in transformation was also observed when bovine serum albumin
(BSA) exposed to ribose for short durations was incubated with plasmid in
absence of the sugar. The effect on DNA was attenuated when excess ribose
remained in the incubation mixture of ribose and protein. The data suggested
that an increase in ribose concentration in the vicinity of DNA could be
damaging and the damage exacerbated, if sugar levels fell subsequently.
Incubation of herring sperm DNA with ribose resulted in a concentration and
time-dependent increase of N2-carboxyethyl-2’-deoxyguanosine (CEdGA,B).
The concentration of CEdGA,B, however, did not increase further when
ribose was removed from the reaction mixture.
 
Date 2010-06-30T09:47:47Z
2010-06-30T09:47:47Z
2010-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/9811
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.47(3) [June 2010]