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Evaluation of suitable solvents for testing the anti-proliferative activity of triclosan - a hydrophobic drug in cell culture

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Title Evaluation of suitable solvents for testing the anti-proliferative activity of triclosan - a hydrophobic drug in cell culture
 
Creator Vandhana, S
Deepa, P R
Aparna, G
Jayanthi, U
Krishnakumar, S
 
Subject Triclosan
Cytotoxicity
Cell culture
Drug sensitivity
Acetone
Polyethylene glycol
 
Description 166-171
Triclosan, a broad spectrum antibiotic is
currently being evaluated for its anti-cancer property. Though several solvents
are available to dissolve lipophilic (hydrophobic) drugs, solubility and
toxicity aspects pose a challenge, when combined with the cell culture medium.
In this paper, we present a simple approach based on physico-chemical and
biologic criteria to choose a suitable solubilizing agent to study the
anti-proliferative property of triclosan in breast cancer cell line MCF-7.
Triclosan was dissolved in five different solvents viz. DMSO, absolute ethanol,
1 N NaOH, 55% polyethylene glycol + 45% ethanol mixture
(PEM) and acetone and diluted with the culture medium
(1 mg/ml). Although triclosan dissolved completely in all five solvents, on
dilution with culture medium, turbidity was observed in DMSO, 1 N NaOH and
ethanol. Cell viability was 95.23% in 10 ml of acetone, when compared with 49.45% at the same volume of PEM.
This non-toxic nature of acetone was supported by DNA fragmentation analysis
and phase contrast microscopy. A significant decrease in cancer cell
proliferation at 100 mg/ml of
acetone-solubilized triclosan, compared with 100 mg/ml of PEM-solubilized triclosan (p<0.05) indicated stronger
anti-proliferative effect and greater drug-sensitivity of triclosan when solubilized
in acetone. Results showed that acetone-solubilized triclosan was suitable for anti-cancer
investigations in cultured MCF-7 cells.
 
Date 2010-06-30T09:55:11Z
2010-06-30T09:55:11Z
2010-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/9814
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.47(3) [June 2010]