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Characterization of seeds of selected wild species of rice (<i style="">Oryza</i>) stored under high temperature and humidity conditions

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Title Characterization of seeds of selected wild species of rice (Oryza) stored under high temperature and humidity conditions
 
Creator Das, Smruti
Nayak, Monalisa
Patra, B C
Ramakrishnan, B
Krishnan, P
 
Subject Oryza
Rice
Wild species
Paddy seeds
Biochemical changes
Proteins
SDS-PAGE
 
Description 178-184
Wild progenitors of rice (Oryza) are an invaluable resource for
restoring genetic diversity and incorporating useful traits back into
cultivars. Studies were conducted to characterize the biochemical changes,
including SDS-PAGE banding pattern of storage proteins in seeds of six wild
species (Oryza alta, O. grandiglumis, O. meridionalis, O.
nivara, O. officinalis and O. rhizomatis) of
rice stored under high temperature (45oC) and humidity (~100%) for
15 days, which facilitated accelerated deterioration. Under the treated
conditions, seeds of different wild rice species showed decrease in per cent
germination and concentrations of protein and starch, but increase in
conductivity of leachate and content of sugar. The SDS-PAGE analysis of seed
proteins showed that not only the total number of bands, but also their
intensity in terms of thickness differed for each species under storage. The
total number of bands ranged from 11 to 22, but none of the species showed all
the bands. Similarity index for protein bands between the control and treated
seeds was observed to be least in O. rhizomatis
and
O. alta, while the indices
were 0.7 and 0.625 for O. officinalis and O. nivara, respectively. This study clearly showed that seed
deterioration led to distinctive biochemical changes, including the presence or
absence as well as altered levels of intensity of proteins. Hence, SDS-PAGE
protein banding pattern can be used effectively to characterize deterioration
of seeds of different wild species of rice.
 
Date 2010-06-30T10:02:21Z
2010-06-30T10:02:21Z
2010-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/9816
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.47(3) [June 2010]