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Adaptation of microsomal glutathione transferase 1 in PC12 cells with modified PMCA isoforms composition

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Title Adaptation of microsomal glutathione transferase 1 in PC12 cells with modified PMCA isoforms composition
 
Creator Boczek, Tomasz
Kozaczuk, Anna
Taha, Joanna
Ferenc, Bozena
Zylinska, Ludmila
 
Subject Plasma membrane Ca2+-ATPase
PC12 cell
Glutathione
Glutathione transferases
Peroxynitrite
 
Description 265-271
Microsomal
glutathione transferase 1 (MGST1) is an integral homo-trimeric membrane protein
with transferase and peroxidase activities. With glutathione as a co-substrate,
it scavenges toxic compounds and may exert anti-apoptotic effect.
We examined the effect of suppression of plasma membrane Ca2+-ATPase
isoforms — PMCA2 or PMCA3 on MGST1 in PC12 cells. GSH level was significantly
higher in PMCA2-reduced line, but similar GSSG/GSH ratios in all cell lines
suggested an efficient protection or absence of oxidative stress. The ATP
concentration decreased in both modified lines, although in PMCA2-suppressed
cells the decrease was higher. Total GSTs activity in postmitochondrial
fraction increased by 30% in the cells with reduced PMCA3. After treatment with
MGST1 activator N-ethylmaleimide (NEM), the activity increased in both
transfected lines by 30-40%. Real-time PCR also showed a higher mRNA expression
of MGST1 in these lines. Staining with antibody recognizing all cytosolic and
membrane-bound GSTs revealed the difference in oligomeric forms of GSTs, and
specific anti-MGST1 antibody showed the presence of MGST1 hexamers in the
transfected cells. Formation of similar hexamers was detected in the control
line after treatment with peroxynitrite. Modification of MGST1 under reduced
PMCAs amount may represent an adaptive mechanism that offers protection against
the cytotoxicity mediated by increased Ca2+
 
Date 2010-11-02T10:26:12Z
2010-11-02T10:26:12Z
2010-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/10519
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.47(5) [October 2010]