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Induction of acute respiratory distress syndrome in rats by lipopolysaccharide and its effect on oxidative stress and antioxidant status in lung

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Title Induction of acute respiratory distress syndrome in rats by lipopolysaccharide and its effect on oxidative stress and antioxidant status in lung
 
Creator Sabarirajan, Jayaraja
Vijayaraj, Panneerselvam
Nachiappan, Vasanthi
 
Subject Acute respiratory distress syndrome
Lipopolysaccharide
Oxidative stress
Alveolar antioxidants
Multiple organ failure
 
Description 278-284
Acute lung injury (ALI) or its severe form,
acute respiratory distress syndrome (ARDS) is an important cause of mortality
in the human population. Despite significant advances made, the mortality
associated with ALI remains unchanged. The objective of the present study was
to evaluate the role of oxidative stress, alveolar antioxidant status and
multiple organ injury in ARDS induced by lipopolysaccharide (LPS) in rats. Rats
were divided into 4 groups, group I control rats were given saline
intraperitoneally, whereas groups II, III and IV (LPS-treated) rats received an
intraperitoneal injection of LPS (10 mg/kg body weight) and sacrificed after
various time intervals. In LPS-treated rats, we observed increased levels of
oxidative products, decreased levels of antioxidants in lung tissues and
increased levels of serum marker enzymes, suggesting multiple organ injury.
Bronchoalveolar lavage fluid (BALF) neutrophil content and protein
concentration in LPS-treated rats were significantly elevated in a
time-dependent manner. Histological studies revealed neutrophil influx and
diffused alveolar damage in LPS-administered rats. These results clearly suggested
that increased oxidant levels led to oxidative stress, antioxidant deficiency
attenuating lung inflammation and tissue damage. LPS administration resulted in
multiple organ failure, leading to increased mortality.
 
Date 2010-11-02T10:26:37Z
2010-11-02T10:26:37Z
2010-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/10521
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.47(5) [October 2010]