Record Details

Biochemical characterization of a calcium-sensitive protein kinase LeCPK2 from tomato

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Biochemical characterization of a calcium-sensitive protein kinase LeCPK2 from tomato
 
Creator Chang, Wenjun
Fu, Gui
Chen, Xin
Zhu, Jiahong
Zhang, Zhili
 
Subject Calcium-dependent protein kinase
LeCPK2
Kinase-Glo® Luminescent Kinase Assay
Tomato
 
Description 148-153
LeCPK2 (GenBank GQ205414),
a versatile calcium-dependent protein kinase (CDPK or CPK) gene was isolated
from tomato in our previous study. In this study, the biochemical properties of
LeCPK2 were further investigated. To examine the role of the C-terminal
calmodulin-like domain (CLD) of LeCPK2 with respect to Ca2+
activation, the kinase activities of recombinant full-length and truncated
LeCPK2 were measured by Kinase-Glo® Luminescent kinase assay
(Promega). The results showed that LeCPK2 activity was Ca2+-dependent
and the C-terminal CLD of 161 residues was essential for
the activation of LeCPK2. The activity of LeCPK2 was sharply stimulated by Ca2+
with K0.5
(concentration of Ca2+ for half-maximal activity) of 48.8 and 45.5
nM with substrate histone IIIs and syntide 2, respectively. The optimal
concentration of Mg2+ for LeCPK2 activity was 20 and 10 mM for substrate histone IIIs and syntide
2, respectively. The Km
value of LeCPK2 towards histone IIIs and syntide 2 was 44.9 μg/ml and 89.52 μM,
respectively. The determination of biochemical properties of LeCPK2 would
provide some clues on how its activity was regulated in vivo.
 
Date 2011-06-16T09:09:32Z
2011-06-16T09:09:32Z
2011-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/11981
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(3) [June 2011]