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A choline monooxygenase gene promoter from Salicornia europaea increases expression of the <img src='/image/spc_char/beta.gif' border=0>-glucuronidase gene under abiotic stresses in tobacco (Nicotiana tabacum L.)

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Title A choline monooxygenase gene promoter from Salicornia europaea increases expression of the -glucuronidase gene under abiotic stresses in tobacco (Nicotiana tabacum L.)
 
Creator Wu, Song
Su, Qiao
An, Lijia
Ma, Saijian
 
Subject : Abiotic stress
Choline monooxygenase
-Glucuronidase activity
Promoter Salicornia europaea
 
Description 170-174
A 1312 bp 5'
flanking region of Salicornia europaea choline
monooxygenase (SeCMO) gene was isolated using the anchored PCR. To investigate
the mechanism of regulation for this stress-induced gene, the SeCMO
promoter--glucuronidase (GUS) chimeric gene constructs containing five
deletions F1, F2, F3, F4 and F5 were introduced into tobacco (Nicotiana tabacum
L.) by Agrobacterium-mediated
transformation. The functional properties of each promoter fragment were
examined by assaying GUS activity in the leaves of transgenic tobacco treated
with abiotic stresses (NaCl, PEG6000 and low temperature). The GUS activity in
transgenic tobacco with F2 (-1056 to +8) construct showed highest increase
under all the three abiotic stresses. Thus, the study provided a potential
promoter induced by the salt, dehydration and cold for the plant genetic
manipulation.
 
Date 2011-06-16T09:12:13Z
2011-06-16T09:12:13Z
2011-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/11985
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(3) [June 2011]