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Involvement of neuronal NO synthase in collateral artery growth

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Title Involvement of neuronal NO synthase in collateral artery growth
 
Creator Pagel, J-I
Borgolte, J
Hoefer, I
Fernández, B
Schaper, W
Deindl, E
 
Subject Neuronal NOS
Arteriogenesis
NO
Collateral artery growth
 
Description 270-274
To evaluate the role of neuronal nitric
oxides synthase (nNOS) in collateral artery growth (arteriogenesis), we analyzed
the expression pattern of nNOS at distinct time points on RNA and protein
levels in a rabbit and a murine model of peripheral arteriogenesis. In the
rabbit model, Northern blot analyses revealed a significant upregulation of
nNOS at 6 h (1.6-fold), 12 h (2.2-fold) and 24 h (2.0-fold) after induction of
arteriogenesis via femoral artery
ligation, when compared to the sham operated side. In mice, an upregulation of
nNOS was also detected using Northern blot (at 6 h, 12 h) and qRT-PCR (12 h:
2.4-fold). On the protein level, nNOS was found to be upregulated 24 h after
femoral artery ligation. Immunohistochemical staining showed that nNOS was localized
in endothelial and smooth muscle cells of collateral arteries, as well as in
skeletal muscle and nerves. In summary, our data provide evidence that nNOS is
not constitutively expressed, but is induced during arteriogenesis, playing a
role in supplying reactive oxygen species such as H2O2 and

low levels of NO.
 
Date 2011-09-16T04:39:02Z
2011-09-16T04:39:02Z
2011-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/12702
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(4) [August 2011]