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Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of <img src='/image/spc_char/alpha.gif' border=0>-smooth muscle actin and transforming growth factor-<img src='/image/spc_char/beta.gif' border=0>1

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Title Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of -smooth muscle actin and transforming growth factor-1
 
Creator Wang, Hong-yue
Wang, Yan-jun
Cui, Ming-ji
Gu, Chun-mei
Yang, Li-zhi
Zhao, Ying
Chen, Yan
Zhao, Dan
Li, Tian-shu
Chi, Bao-rong
 
Subject PCI-neo-hepatocyte growth factor
5/6 Nephrectomized rats
Transforming growth factor-1
-Smooth muscle actin
 
Description 308-315
Several studies have shown that hepatocyte
growth factor (HGF) ameliorates renal interstitial fibrosis, but the mechanism
is not fully clear. This study was designed to examine whether HGF can relieve
renal interstitial injury in 5/6 nephrectomized rats, and to confirm whether this
function was associated with decrease in -smooth muscle actin (-SMA) and
transforming growth factor-beta1 (TGF-1) expression. The animals were
randomized into 8 groups comprising 6 animals (n = 6) each: control (group I),
PCI-neo (group II, 900 μg), sham-operation (group III，not nephrectomy), model or 5/6 nephrectomy group (group IV),
lotensin group (an angiotensin converting enzyme inhibitor, group V, 0.6 mg/100
g/day for 5 weeks), low-dose PCI-neo-HGF group (group VI, 690 μg), high-dose
PCI-neo-HGF group (group VII, 1380 μg) and lotensin + high-dose PCI-neo-HGF
group (group VIII, 0.6 mg/100 g/day for 5 weeks, 1380 μg). The animals were
sacrificed in the 5th week after 5/6 nephrectomy. The specimens of
kidneys were used for pathological examination
(hematoxylin-eosin staining), detection of -SMA and TGF-1 mRNA
(Reverse transcriptase-polymerase chain reaction) and protein (Western blot and
immunohistochemistry)
expression. The results showed that in 5/6 nephrectomized rats blood urea
nitrogen (BUN), serum creatinine (CRE) and 24 h urinary albumin excretion (UAE)
were increased, renal interstitium was injured seriously and -SMA, TGF-1 mRNA
and protein expression were elevated compared with those of control. The above
changes were ameliorated and -SMA and TGF-1 expression was reduced by both
PCI-neo-HGF and lotensin. The lotensin + high-dose PCI-neo-HGF group rats
exhibited the most significant therapeutic effect both in decreasing the BUN,
CRE and 24 h UAE and in relieving renal interstitial injury. In conclusion, the
study demonstrated that HGF can relieve renal interstitial injury and this
protection was associated with down-regulation of –SMA and TGF-1
expressions.
 
Date 2011-10-21T11:00:54Z
2011-10-21T11:00:54Z
2011-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/12938
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(5) [October 2011]