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Role of chlorophyllase in chlorophyll homeostasis and post-harvest breakdown in <i>Piper betle</i> L. leaf

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Title Role of chlorophyllase in chlorophyll homeostasis and post-harvest breakdown in Piper betle L. leaf
 
Creator Gupta, Supriya
Gupta, Sanjay Mohan
Kumar, Nikhil
 
Subject Piper betle
Degradation
Chlorophyllase
Chlorophyllide
Leaf senescence
Hormones
 
Description 353-360
Piper betle L., a dioecious shade-loving perennial climber is one
of the important Pan-Asiatic plants. More than hundred landraces having marked
variation in leaf chlorophyll (Chl) content are in cultivation in India. In
this study, role of chlorophyllase (Chlase) in Chl homeostasis and post-harvest
breakdown was investigated in two contrasting P. betle landraces Kapoori
Vellaikodi (KV) with light green and Khasi Shillong (KS) with dark green
leaves. The two landraces showed negative correlation between Chl content and
Chlase activity in fresh as well as stored leaves. Accumulation of
chlorophyllide a (Chlid a) was correlated with the level of Chlase
activity, which was higher in KV than KS. The overall response of abscisic acid
(ABA) and benzylaminopurine (BAP) was similar in KV and KS, however, the
time-course was different. ABA-induced Chl loss was accompanied by rise in
Chlase activity in KV and KS and the delay in Chl loss by BAP was accompanied
by reduction in Chlase activity. While there were significant differences in
Chlase activity in KV and KS, only minor differences were observed in the
enzyme properties like pH and temperature optima, Km and Vmax.
No landrace-related differences were observed on the effect of metal ions and
functional group reagents/amino acid effectors on Chlase activity. These
results showed that despite significant differences in Chl content and Chlase
activity between landraces KV and KS, the properties of Chlase were similar.
The findings show that in P. betle Chlase is involved in
Chl homeostasis and also in Chl degradation during post-harvest storage and
responds to hormonal regulations.
These findings might be useful in predicting the stability of Chl during post-harvest
storage and also the shelf-life in
other P. betle landraces.
 
Date 2011-10-21T11:04:45Z
2011-10-21T11:04:45Z
2011-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/12945
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(5) [October 2011]