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<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Functional fusion expression of sunflower multicystatin in <i>E. coli</i> and its comparison with a single domain cystatin</span>

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Title Functional fusion expression of sunflower multicystatin in E. coli and its comparison with a single domain cystatin
 
Creator Gholizadeh, Ashraf
Kohnehrouz, B Baghban
 
Subject Cystatin
Expression
Fusion protein
Inhibitor
Sunflower multicystatin
Phytocystatin
 
Description 375-379
Identification of the molecular structure and novel biophysiological
functions of plant cystatins or phytocystatins is of great interest in the
field of molecular biology. The important
requirements for these are the efficient production, purification and
correctly folded forms of these proteins. We report here the cloning, easy
expression and characterization of a sunflower multicystatin (SMC) as a
functional fusion protein in E. coli. For the first time, the amplified
cystatin coding region was expressed as a part of maltose-binding fusion
protein using pMALc2X over-expression vector in TB1 strain of
E. coli without affecting the recombinant bacterial growth. In
comparison to the previously prepared recombinant SMC (rSMC), a high amount
(~44 mg/L of bacterial cell culture) of purified fused SMC (fSMC) was obtained
using single-step purification method. fSMC strongly inhibited papain activity in
vitro as compared to Celosia single-domain cystatin. Purified
fSMC may be used for basic biochemical, pharmacological or clinical studies
without the cleavage of its fusion parts.
 
Date 2011-12-23T10:28:42Z
2011-12-23T10:28:42Z
2011-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13250
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(6) [December 2011]