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Protective effect of ellagic acid and pumpkin seed oil against methotrexate-induced small intestine damage in rats

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Title Protective effect of ellagic acid and pumpkin seed oil against methotrexate-induced small intestine damage in rats
 
Creator El-Boghdady, Noha A
 
Subject Methotrexate
Oxidative stress
Ellagic acid
Pumpkin seed oil
Purine catabolizing enzymes
Prostaglandin E2
 
Description 380-387
Gastrointestinal
toxicity is one of the most serious side effects in the methotrexate (MTX)
treatment. This study was designed to investigate whether ellagic acid (EA)
and/or pumpkin seed oil (PSO) had a protective effect on MTX-induced small intestine
damage. Forty albino rats were randomized into five groups of 8 rats each.
Group II, MTX was administered as a single dose (20
mg/kg) intraperitoneally. Groups IV and V were pre-treated respectively with
either PSO (40 mg/kg), EA (10 mg/kg) or 0.2% DMSO (vehicle control) orally
every day by gavage for 5 days and then they received MTX. All animals were
sacrificed 5 days after the intraperitoneal injection of MTX for
histopathological examination, estimation of serum prostaglandin E2
(PGE2) level, assay of tissue malondialdehyde (MDA), reduced
glutathione (GSH) and nitric oxide (NO) levels and myloperoxidase (MPO),
xanthine oxidase (XO) and adenosine deaminase (AD) activities. Administration
of EA and/or PSO decreased the intestinal damage, PGE2, MDA and NO
levels and MPO, XO and AD activities and increased GSH level. These results
suggest that EA and PSO protect the small intestine of rats from MTX-induced
damage through their antioxidant and anti-inflammatory effects and thus have
potential as a promising drug in the prevention of undesired side effects of
MTX.
 
Date 2011-12-23T10:29:17Z
2011-12-23T10:29:17Z
2011-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13252
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(6) [December 2011]