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Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance

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Title Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance
 
Creator Lee, Jung Hun
Sohn, Seung Ghyu
Jung, Ha Il
An, Young Jun
Lee, Jae Jin
Kang, Lin-Woo
Lee, Sang Hee
 
Subject Antibiotic resistance
Imipenem
Crystal
OXA-23
Carbapenemase
Acinetobacter baumannii
 
Description 395-398
OXA-23, a class D carbapenemase that confers widespread antibiotic
resistance hydrolyzes the β-lactam
rings of
β-lactam antibiotics, presenting an enormous challenge to infection control, particularly in
the eradication of pathogenic bacteria such as Acinetobacter baumannii, one of six top-priority dangerous
pathogens. Thus, the enzyme is a potential target for developing antimicrobial
agents against pathogens producing carbapenemases. In this study, OXA-23 was
purified and crystallized at 298 K and X-ray diffraction data from OXA-23 crystal
were collected at 2.03 Å resolution using synchrotron radiation. The crystal of
OXA-23 belonged to space group P41
with unit cell parameters a = 82.47, b = 82.47 and c = 172.01 Å. Analysis of the packing density showed that the
asymmetric unit probably contained two molecules with a solvent content of 73.64%.
 
Date 2011-12-23T10:32:00Z
2011-12-23T10:32:00Z
2011-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13255
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(6) [December 2011]