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<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"DejaVu LGC Sans";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:#00FF;mso-bidi-language: #00FF" lang="EN-GB">SAD phasing with in-house Cu <i style="mso-bidi-font-style:normal">Kα </i>radiation using barium as anomalous scatterer</span>

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Title SAD phasing with in-house Cu Kα radiation using barium as anomalous scatterer
 
Creator Dhanasekaran, V
Velmurugan, D
 
Subject Lysozyme
In-house phasing
Single wavelength anomalous diffraction
Redundancy
 
Description 399-405
Phasing of lysozyme
crystals using co-crystallized barium ions was performed using
single-wavelength anomalous diffraction (SAD) method using Cu Kα radiation with in-house source of
data collection. As the ion binding sites vary with respect to the pH of the
buffer during crystallization, the highly isomorphic forms of lysozyme crystals
grown at acidic and alkaline pH were used for the study. Intrinsic sulphur
anomalous signal was also utilized with anomalous signal from lower occupancy
ions for phasing. The study showed that to solve the structure by SAD
technique, 2.8-fold data redundancy was sufficient when barium was used as an
anomalous marker in the in-house copper X-ray radiation source for data
collection. Therefore, co-crystallization of proteins with barium containing
salt can be a powerful tool for structure determination using lab source.
 
Date 2011-12-23T10:32:29Z
2011-12-23T10:32:29Z
2011-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13256
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.48(6) [December 2011]