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Purification and characterization of phytase with a wide pH adaptation from common edible mushroom <i style="">Volvariella volvacea</i> (Straw mushroom)

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Title Purification and characterization of phytase with a wide pH adaptation from common edible mushroom Volvariella volvacea (Straw mushroom)
 
Creator Xu, Lijing
Zhang, Guoqing
Wang, Hexiang
Ng, Tzi Bun
 
Subject Straw Mushroom
Phytase
Isolation
Volvariella Volvacea
 
Description 49-54
A novel phytase with a
molecular mass of 14 kDa
was
isolated from fresh fruiting bodies of the common edible mushroom Volvariella volvacea (Straw mushroom).
The isolation procedure involved successive chromatography on DEAE-cellulose, CM-cellulose,
Affi-gel blue gel, Q-Sepharose and Superdex-75. The enzyme was a monomeric protein and was
unadsorbed on DEAE-cellulose, CM-cellulose and Affi-gel blue gel, but was adsorbed on Q-Sepharose. The enzyme was purified
51.6-fold from the crude extract with 25.9% yield. Its N-terminal amino acid
sequence GEDNEHDTQA exhibited low homology to
the other reported phytases. The optimal pH and temperature of the purified enzyme was 5 and 45oC, respectively. The enzyme was quite stable over the pH
range of 3.0 to 9.0 with less than 30% change in its activity, suggesting that
it can be used in a very wide pH range. The enzyme exhibited broad substrate selectivity
towards
various phosphorylated compounds, but lacked antifungal activity against tested plant pathogens.
 
Date 2012-02-14T12:28:22Z
2012-02-14T12:28:22Z
2012-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13591
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.49(1) [February 2012]