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Inhibitory effect of antibodies against human chorionic gonadotropin on the growth of colorectal tumour cells

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Title Inhibitory effect of antibodies against human chorionic gonadotropin on the growth of colorectal tumour cells
 
Creator Khare, Priyanka
Singh, Om
Jain, Swatantra K
Javed, Saleem
Pal, Rahul
 
Subject Colorectal Cancer
Human Chorionic Gondotropin
Immunotherapy of Cancer
 
Description 92-96
Human chorionic gonadotropin (hCG) was initially believed to be secreted
exclusively by the embryo with its primary function being “rescue” of the
corpus luteum. However, recently it has been found that the hormone (or its
individual subunits) is also secreted by many cancers and that in many cases
secretion is associated with poor patient prognosis. In this study, we assessed
the presence of hCG in colorectal cancer cells (CCL-253) and evaluated the
anti-tumour effects of
anti-hCG antibodies in vitro and in vivo. Anti-hCG antibodies were
reactive with CCL-253, as revealed by confocal immunoflourescence microscopy;
both cell surface and intracellular expression were observed. Western blot
analysis showed that antibodies appeared to interact with several moieties,
indicating a level of cross-reactivity. Anti-hCG antiserum specifically reduced
the viability of tumor cells and the addition of complement increased in vitro anti-tumor effects. In nude
mice implanted with CCL-253 cells, administration of anti-hCG antiserum caused
a significant reduction in tumor volume; all treated animals survived, while mortality
was observed in control animals. Results suggest that anti-hCG antibodies can
mediate significant anti-tumor activity both in vitro and in vivo and
lend support to the rationale of anti-hCG immunization in the therapy of
gonadotropin- sensitive cancers.
 
Date 2012-04-17T08:53:35Z
2012-04-17T08:53:35Z
2012-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/13839
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.49(2) [April 2012]