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Depolymerization of starch and pectin using superporous matrix supported enzymes

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Title Depolymerization of starch and pectin using superporous matrix supported enzymes
 
Creator Lali, Arvind
Manudhane, Kushal
Motlekar, Nuzhat
Karandikar, Priti
 
Description 253-258
Immobilized enzyme catalyzed biotransformations involving
macromolecular substrates and/or products are greatly retarded

due to slow diffusion of large substrate molecules in
and out of the typical enzyme supports. Slow diffusion of macromolecules into
the matrix pores can be speeded up by use of macroporous supports as enzyme
carriers. Depolymerization reactions of polysaccharides like starch, pectin,
and dextran to their respective low molecular weight products are some of

the reactions that can benefit from use of such
superporous matrices. In the present work, an indigenously prepared rigid cross-linked
cellulose matrix (called CELBEADS) has been used as support for immobilizing
alpha amylase (1,4-α-Dglucan glucanohydrolase, EC 3.2.1 . 1.) and pectinase
(endo-PG: poly( 1 ,4-α-galactouronide) glycanohydrolase, EC 3.2. 1.1 5).

The immobilized enzymes were used for starch and
pectin hydrolysis respectively, in batch, packed bed and expanded bed

modes. The macroporosity of CELBEADS was found to
permit through-flow and easy diffusion of substrates pectin and

starch to enzyme sites in the porous supports and gave
reaction rates comparable to the rates obtained using soluble enzymes.
 
Date 2012-12-22T19:57:45Z
2012-12-22T19:57:45Z
2002-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15268
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR, CSIR
 
Source IJBB Vol.39(4) [August 2002]