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Immunoaffinity layering enhances the sensitivity of antigen detection on nitrocellulose strips

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Title Immunoaffinity layering enhances the sensitivity of antigen detection on nitrocellulose strips
 
Creator Jamil, Hina
Saleemuddin, Mohammed
 
Description 274-278
A simple strategy to remarkably increase the sensitivity
of detection of antigens applied as dot or western blot on nitrocellulose
membrane using human serum albumin as model antigen has been described. This
involves subjecting the antigen bearing nitrocellulose strips to multiple
incubation cycles with primary antibody and enzyme conjugated secondary

antibody prior to staining for enzyme activity. The
sensitivity of detection could be increased up to a thousand fold after three
incubation cycles. Aggregation of human serum albumin could be detected by the
multiple incubation procedure at very low protein concentration after
electrophoresis and transfer onto nitrocellulose.
 
Date 2012-12-22T19:59:51Z
2012-12-22T19:59:51Z
2002-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15271
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(4) [August 2002]