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Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis

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Title Resolution of a complex ionic mixture of an apparently homogenous protein preparation by preparative electrophoresis
 
Creator Dubey, Ashok K
 
Description 279-283
Preparations of recombinant envelope glycoprotein E2
of hepatitis C virus (r-HCV E2), found to be homogeneous by N-terminal amino
acid sequencing and mass spectrometry, resolved into multiple ionic species
(isoforms) when analysed by isoelectric focusing (IEF) gel electrophoresis in
the pI range of 3-10. These isoforms
possessed pI  values in the range or

4.5-8.2. The major isoform with pI value of approximately 7. 1 was separated from the rest of them
by employing a method developed on Gradiflow BF 200, a device based on
preparative electrophoresis. This isoform was adjudged to be homogenous by IEF
and by native polyacrylamide gel electrophoresis (PAGE).
 
Date 2012-12-22T20:00:21Z
2012-12-22T20:00:21Z
2002-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15272
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(4) [August 2002]