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Purification and properties of invertase from the flowers of <i>Woodfordia fruticosa</i>

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Title Purification and properties of invertase from the flowers of Woodfordia fruticosa
 
Creator Weersaooriya, M K B
Yatawara, H P
 
Description 347-350
Invertase (β- fructofuranosidase, EC 3.2.1.26) was purified

from the  flowers of of
 Woodfordia fruticosa,  which is used to prepare

certain fermented Ayurvedic drugs.
The enzyme was purified to near homogeneity as judged by native PAGE with a
yield of 10.7%, using

(N H4)2SO4
fractionation, followed by gelfiltration through Sepharose 4B and DEAE
cellulose chromatography at pH 6.8
and 4.42. The molecular mass of the purified enzyme as determined by elution
through Sepharose 4B gel column was found to be ~ 280 kDa. SDS-PAGE of the
purified enzyme showed that the enzyme is composed of three subunits with
molecular mass of  66. 43 and 40 kDa. The
enzyme showed a broad pH optimum between
4.0-7.0. Optimum assay temperature was 37°C and above 45°C, the enzyme activity
slowly declined and inactivated around 80°C. The apparent Km value
of th e enzyme for sucrose was 160mM.
 
Date 2012-12-08T16:15:45Z
2012-12-08T16:15:45Z
2002-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15212
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher CSIR
 
Source IJBB Vol.39(5) [October 2002]