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Modulation of α1 β1 integrin mediated adhesion of hepatocytes to collagen IV and laminin by divalent cations

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Title Modulation of α1 β1 integrin mediated adhesion of hepatocytes to collagen IV and laminin by divalent cations
 
Creator Menon, Bindu
Kumar, N Anil
Sudhakaran, P R
 
Description 159-165
Cell matrix interactions play a critical role in
hepatic development and regeneration after acute injury. These interactions are
mediated by transmembrane receptors belonging mainly to the integrin family. We
have tried to assess the role of divalent cations in mediating attachment of
hepatocytes to matrix proteins like collagen IV (Col IV) and laminin

(Ln). The three cations examined viz. Ca2+,
Mg2+ and Mn2+ showed attachment promoting activity. Since
α1 β1 integrin is a common receptor for col IV and LN in
liver, the effect of cations in its binding to these matrix proteins was
studied. Although cations in general enhanced the binding, different cations
exhibited differential effect in promoting the binding for

different ligands. Mg2+ ions were more
effective in promoting the binding of  α1
β1 integrin to col IV but Ca2+ proved to be more effective one for Ln. Kinetic
analysis of binding in dot blot assays using different concentrations of
cations showed that while Mg2+ was active at low concentrations Ca2+ and Mn2+
promoted the binding more at higher concentrations. Absence of competitive
effect in binding studies showed that they bind at different sites on the
receptor. Differential effects of cations in promoting the binding of  α1 β1 integrin to Col IV
and Ln suggest that changes in level of diffusible cations can modulate
affinity of the common receptor α1 β1
integrin to its ligands and can influence
adhesion of hepatic cells to different

matrix proteins during hepatic development and
regeneration.
 
Date 2012-12-24T18:39:31Z
2012-12-24T18:39:31Z
2001-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15288
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(3) [June 2001]